SYSTEMATIC BACTERIOLOGY

                                                        SYSTEMATIC BACTERIOLOGY SYLLABUS

THEORY
Aims and Objectives: This subject will give information about the different types of bacterial culture procedures,
staining procedures and Biochemical tests used for identification of bacteria. The students will learn the
morphology cultural characteristics, biochemical characteristics & laboratory diagnosis of various bacteria.

Suggested Readings:
1. Practical Medical Microbiology by Mackie &MacCartney Volume 1 and
2. Text book of Microbiology by Ananthanereyan
3. Medical Microbiology by Paniker&Satish Gupte
4. Medical laboratory Technology vol.I ,II, III by Mukherjee
5. Medical Laboratory manual for tropical countries Vol II Microbiology by MoniaCheesbrough

1. Bacterial culture a. Instruments used to seed culture media b. Culture procedures – seeding a plate
2. Staining techniques in bacteriology Principle, procedures, significance and interpretation of the following
staining techniques: Simple staining, Gram stain, Ziehl –Neelsen staining, Albert’s stain, Capsule staining.
3. Principle, procedures and interpretation of the following biochemical tests for identification of different
bacteria: Catalase, Coagulase, Indole, Methyl Red, VogesProskauer, Urease, Citrate, Oxidase, TSI, Nitrate
reduction, Carbohydrate fermentation, Huge and leifson, Bile solubility, H2 S production, Demonstration of
motility
4. Various characteristics (morphological, cultural and biochemical), pathogenesis and laboratory diagnosis of
the following bacteria a) Staphylococcus b) Streptococcus c) Pneumococus d) Neisseria gonorhoeae and
Neisseria meningitis e) Haemophilis f) Corynebacterium g) Enterobacteriaceae: Escherichia coli, Klebsiella,
Proteus , Salmonella, Shigella h) Vibrio i) Clostridium j) Mycobacterium tuberculosis complex, Atypical
Mycobacteria and M. leprae k) Spirochetes – Treponema, Borrellia and leptospira l) Bordetella and brucella m)
Mycoplasma and Ureaplasma