BMLS103-18- BASICS OF BIOCHEMISTRY
Theory
Aims /learning Objectives: The main objective of the subject is to impart the knowledge of apparatus, units,
equipments, volumetric analysis in the laboratory of clinical Biochemistry.
1. Introduction to Medical lab Technology. (a) Role of Medical lab Technologist, (b) Ethics and responsibility,
(c) Safety measures (d) First aid.
2. Cleaning and care of general laboratory glass ware and equipments. (a) Steps involved in cleaning soda
lime glass (b) Steps involved in cleaning borosil glass. (c) Preparation of chromic acid solution. (d) Storage.
3. Distilled water. (a)Method of preparation of distilled water (b) Type of water distillation plants (c)Storage of
distilled water
4. Units of Measurement. (a) S.I unit and CGS units (b) Conversion (c) Strength, molecular weight, equivalent
weight (d)Normality, Molarity, Molality (e) Numericals.
5. Calibration of volumetric apparatus (a) Flask (b) Pipettes (c) Burettes (d) Cylinders
6. Analytical balance (a) Principle (b) Working (c) Maintenance
7. Concept of pH (a) Definition (b) Henderson Hasselbatch equation (c) Pka value (d) pH indicator (e) Methods
of measurement of pH (i) pH paper (ii) pH meter (iii)Principle, working, maintenance and calibration of pH
meter
8. Volumetric analysis (a) Normal and molar solutions (b) Standard solutions (c) Preparation of reagents
(d)Storage of chemicals
Course Features
- Lectures 25
- Quizzes 0
- Duration 10 weeks
- Skill level All levels
- Language English
- Students 5
- Assessments Yes