GENERAL MICROBIOLOGY

MLS102-18- GENERAL MICROBIOLOGY
Theory
Aims /learning Objectives: This subject gives the general insight into history and basics of medical
microbiology, imparts the knowledge about equipments used in Medical Microbiology and basic
procedures done in medical microbiology laboratory i.e. microscopy, sterilization, disinfection, culture
methods required to perform different microbiological tests in clinical microbiology lab and biomedical
waste management.
1. Introduction to Medical Microbiology: Definition – History – Host-Microbe relationship.
2. Safety Measures in clinical microbiology
3. Glassware used in Clinical Microbiology Laboratory:Introduction – Care and handling of glassware –
Cleaning of glassware
4. Equipments used in clinical Microbiology Laboratory: Introduction – Care and maintenance
5. Microscopy: Introduction and history, Types of microscope, (a) Light microscope (b) DGI(c) Fluorescent
(d) Phase contrast (e) Electron microscope: Transmission, Scanning, Principles and operational mechanisms
of various types of microscopes
6. Sterilization: Definition,Types and principles of Sterilization, method (a) Heat (dry heat, moist heat with
special Reference to autoclave (b) Radiation (c) Filtration, Efficiency testing to various sterilizers
7. Antiseptics and disinfectants: Definition: Types and properties – Mode of action – Uses of various
disinfectants, Precautions while using the disinfectants – Qualities of a good disinfectant, In-house
preparation of alcoholic hand/skin disinfectant, Testing efficiency of various
8. Biomedical waste management in a Microbiology lab: Types of the waste generated – Segregation –
Treatment – Disposal.
9. General characteristics & classification of Microbes : (Bacteria & fungi): Classification of microbes
with special reference to prokaryotes & eukaryotes, Morphological classification of bacteria, Bacterial
anatomy (Bacterial cell structures)
10. Growth and Nutrition of Microbes: General nutritional & other requirements of the bacteria, Classification
of bacteria on the basis of their nutritional requirements, Physical conditions required for growth, Normal
growth cycle of bacteria (growth Curve), Types of microbial cultures: Synchronous, Static,
continuousculture
11. Culture media: Introduction, Classification of culture media ( Example & Uses ) solid media, liquid media,
semisolid, Media,, routine/synthetic/defined media, basal media, enriched , enrichment, Selective,
differential media, sugar fermentation media, transport media, preservation media and anaerobic culture
media.
12. Aerobic & anaerobic culture methods: Concepts, Methods Used for aerobic cultures, Methods Used for
anaerobic cultures